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Files in this zip folder: AmyMLUtility.py - implements the dataset class ImageDataset AmyMLUtility.html - ImageDataset class documentation DatasetExample.py - a simple program to demonstrate ImageDataset form.py - a module stolen from another project that implements a dead-simple GUI parameter input and validation form.html - documentation for form.py Image_Simulation_NNTraining.m - image simulation script slightly modified for use with ImageDataset form.py needs PyQt5. If you have anaconda installed as recommended with spyder in your conda project environment, this is already installed, otherwise conda install -c anaconda pyqt in you anaconda project environment AmyMLUtility.py need opencv/cv2, to install it conda install -c conda-forge opencv apparently this opencv does not include the support for actual videos like .avi, we don't need it for this application note that you can use the SimpleGUI class in form.py to do input to matlab functions/scripts by using the python matlab.engine python library